Supplementation of Zinc and Vitamin E on Apparent Digestibility of Nutrient, Carcass Traits, and Mineral Availability in Broiler Chickens

Tera Fit Rayani, R. Mutia, Sumiati Sumiati


The purpose of this study was to determine the effect of zinc (Zn) and vitamin E supplementations on apparent nutrient digestibility, carcass traits, and mineral availability of broiler in tropical environment. The experiment was arranged in a 3 x 3 factorial design with 4 replications in each treatment (10 birds each). The dietary treatments were the combination of doses of zinc (A1: 0 ppm, A2: 40 ppm, A3: 80 ppm) and vitamin E (B1: 0 ppm, B2: 125 ppm, B3: 250 ppm) supplementations. The variables observed were apparent nutrient digestibilities (apparent crude protein digestibility and apparent ether extract digestibility), carcass traits (final weight, the percentage of carcass weight, breast weight, thigh weight, wings weight, and back weight), meat bone ratio and mineral availability in the tibia (calcium [Ca], phosphorus [P], and zinc [Zn]). The results showed that apparent nutrient digestibility, carcass traits (the percentages of carcass, breast, thighs, wings, and back), meat bone ratio were not significantly influenced by the supplementation of zinc or vitamin E. The supplementation of 80 ppm of Zn and 0 ppm of vitamin E significantly (P<0.05) increased final weight of broiler. Supplementation of zinc alone and vitamin E alone in the diet significantly (P<0.05) increased calcium content of the tibia. In conclusion, supplementation of 80 ppm of Zn increased calcium content compared to control, but did not affect final weight and ether-extract digestibility. Supplementation of vitamin E at a dose of 125 ppm could increase calcium content of the tibia compared to control.


broiler; vitamin E; zinc

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AOAC. 2005. Official Methods of Analysis of Association of Official Analitycal Chemists. 16th ed. Assoc. Off. Anal. Chem., Arlington.

[BPS] Badan Pusat Statistik. 2015. Konsumsi rata-rata per kapita seminggu beberapa macam bahan makanan penting 2007-2014. . [8 August 2016]

Burkholder, K. M, K. L. Thompson, . E. Einstein, T. J. Applegate & J. A. Patterson. 2008. Influence of stressors on normal intestinal microbiota, intestinal morphology and susceptibility to Salmonella enteriditis colonization in broilers. Poult. Sci. 87:1734-1741.

Farrel, D. J. 1978. A nutritional evaluation of buckwheat (Fagopyrum esculentum). Anim. Feed. Sci. Technol. 3(2):95-108.

Gu, X. H, Y. Hao & X. L. Wang. 2012. Over-expression of heat shock protein and its relationship to intestine under acute heat stress in goat. Int. J. Livest. 3: 27-28.

Habibian, M, S. Ghazi , M.M. Moeini & A. Abdolmohammadi. 2014. Effect of dietary selenium and vitamin E on immune response and bioligical blood parameters of broiler reared under thermoneutral or heat stress condition. Int. J. Biomet. 58(5): 741-752.

Hao, Y, X. H. Gu & X. L. Wang. 2012. Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers: 1. Intestinal structure and digestive function. Poult. Sci. 91:781-789.

Hashizawa, Y, M. Kubota, M. Kadowaki & S. Fujimura. 2013. Effect of dietary vitamin E on broiler meat qualities, color, water-holding capacity and sher force value under heat stress condition. Anim. Sci. J. 80(11): 732-736.

Hosseini-Vashan, S. J., A. Golian, & A. Yaghobfar. 2016. Growth, immune, antioxidant, and bone responses of heat stress-expossed broilers fed diets supplemented with tomato pomace. Int. J. Biometeorol. 60:1183-1192.

Huang, Y. L, L. Lu, X. G. Luo & B. Liu. 2007. An optimal dietary zinc level of broile chicks fed a corn-soybean meal diet. Pout. Sci. 86:2582-2589.

Khakhi, R. A. M, R. Bakhshalinejad, A. Hassanabadi & P. Ferket. 2016. Effect of dietary organic zinc and α-tocopheryl acetate supplements on growth performance,, meat quality, tissue minerals, and α-tocopherol deposition in broiler chickens. Poult. Sci. Poult. Sci. pew386.

Lara, L. J & M. H. Rostagno. 2013. Impact of heat stress on poultry production. Animals. 3:356-369.

Leeson, S & J. D. Summers. 2008. Comercial Poultry Nutrition. 3rd ed. Department of Animal and Poultry Science, University of Guelph. University Books., Guelph.

Liu, F, J. Yin, M. Du, P. Yan, J. Xu, X. Zhu, & J. Yu. 2009. Heat-stress-induced damage to porcine small intestineal ephitelium associated with downregulation of epithelial growth factor signaling. J. Anim. Sci. 87:1941-1949.

Liu, Z, L. Lu, S. Li, Z. Zhang, L. Xi, K. Zhang, & X. Luo. 2011. Effects of supplemental zinc source and level on growth performance, carcass traits and meat quality of broilers. Poult. Sci. 90:1782-1790.

Liu, Z. H, L. Lu, R. L. Wang, . L. Lei, S. F. Li, L. Y. Zhang, & X. G. Luo. 2015. Effects of supplemental zinc source and level on antioxidant ability and fat metabolism-related enzymes of broilers. Poult. Sci. 94: 2686-2694.

Lohakare, J. D, Y. J. Choi, J. K. Kim, J. S. Yong, Y. H. Shim, T. W. Hahn, & B. J. Chae. 2005. Effect of dietary combinations of viramin A, E, methionine on growth performance, meat quality and immunity in comercial broilers. J. Anim. Sci. 18(4): 516-523.

Mansoub, N. H, S. C. Azar, A. A. Tehrani, A. Lotfi, & M. K. Manesh. 2010. Influence of dietary vitamin E and zinc on performance, oxidative stability and some blood measures of broiler chickens reared under heat stress. J. Agrobiol. 27(2): 103-110.

Onderci, M, N. Sahin, K. Sahin, & N. Kilic. 2003. The antioxidant properties of chromium and zinc: in vivo effects on digestibility, lipid peroxidation, antioxidant vitamins and some minerals under a low ambient temperature. Biol. Trace. Elem. Res. 92: 139-150.

Quinteiro-Filho, W. M, A. Riberio, V. Ferraz-de-Paula, M. L. Pinheiro, M. Sakai, L. R. M. Sa, A. J. P. Ferreira, & J. Palermo-Neto. 2010. Heat stress impairs performance parameters, induces intestinal injury, and dcreases macrophage activity in broiler chickens. Poult. Sci. 89:1905-1914.

Rossi, P, F. Rutz , M. A. Anciuti, J. L. Rech, & N. H. F. Zauk. 2007. Infuence of graded levels of organic zinc on growth performance and carcass traits of broilers.J. Appl. Poult. Res. 16: 219-225.

Rozenboim, I, E. Tajo, O. Gal-Garber, J. A. Proudman, & Z. Uni. 2007. The effect of heat stress on ovarium function of laying hens. Poult. Sci. 86:1760-1765.

Rusli, R. K, K. G Wiryawan, T. Toharmat, Jakaria, & R. Mutia. 2015. Effect of manggosteen pericarp meal and vitamin E supplements on the performance, blood profiles, antioxidant enzyme and HSP 70 gene expression of laying hens in tropical environment. Int. J. Poult. Sci. 14(10):570-576.

Sahin, K & O. Kucuk. 2001. Effects of vitamin E and selenium on performance, digestibility of nutrients, and carcass characteristics of Japanese quails reared under heat stress (34 ºC). J. Anim. Physiol. a. Anim. Nutr. 85: 342-348.

Sahin, K & O. Kucuk. 2003. Heat stress and dietary vitamin supplementation of poultry diets. Nutr. Abstr. Rev. Ser. B. Livest. Feeds. Feeding. 73: 41-50.

Sahin, K, M. Onderci, N. Sahin, T. A Balci, M. F Gursu, V. Juturu, & O. Kucuk. 2006. Dietary arginine silicate inositol complex improves bone mineralization in quail. Pout. Sci. 85:486-492.

Sahin, N, C. Orhan, M. Tuzcu, K. Sahin, & O. Kucuk. 2008. The effect of tomato powder supplementation on performance and lipid peroxidation in quail. Poult Sci. 87:267-283.

Sahrei, M, H. Janmmohamadi, A. Taghizadeh, & S. Cheraghi. 2012. Effect of different zinc sources on tibia bone morphology and ash content of broiler chickens. Advan. Biol. Res. 6(3): 128-132.

Salim, H. M, C. Jo, & B. Lee . D. 2008. Zinc in broiler feeding and nutrition. Avian. Bio. Res. 1: 5-18.

Salim, H, H. Lee, C. Jo, S. Lee, & B. Lee. 2012. Effect of dietary zinc proteinate supplementation on growth performance and skin and meat quality of male and female broiler chicks. Br. Poult. Sci. 53:116-124.

Selim, N. A, S. F. Youssef, A. F. Abdel-Salam, & S. A. Nada. 2013. Evaluation of some natural antioxidant sources in broiler diets: 1-effect on growth, physiological, microbiological and immunological performance of broiler chicks. Int. J. Poult. Sci. 12:561-571.

Sohail, M. U, M. E. Hume, J. A. Byrd, D. J. Nisbet, A. Ijaz, A. Sohail, M. Z. Shabbir, & H. Rehman. 2012. Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to cronic heat stress. Poult. Sci. 91:2235-2240.

Star, L. B. Kemp. I. Van den Anker, & H. K. Parmentier. 2008. Effect of single or combined climatic and hygienic stress in four layer lines: 1. Performance. Poult. Sci. 87:1022-1030.

Steel, R. G. D & J. H. Torrie. 1995. Prinsip dan Prosedur Statistika. Suatu pendekatan Biometrik. Terjemahan. 5th ed. Gramedia Pustaka Utama, Jakarta.

Sudarman, A, Sumiati, & S. H. Solikhah. 2011. Performance and meat cholesterol content of broiler chickensfed Pluchea indica L. leaf meal reared under stress condition. Med. Pet. 34:64-68.

Sunder, G. S, A. K. Panda, N. C. S. Gopinath, S. V. Rama Rao, M. V. L. N. Raju, M. R. Reddy, & K. Ch. Vijay. 2008. Effect of higher levels of zinc supplementation on performance, mineral availability and immune competence in broiler chickens. J. Appl. Poult. Res. 17: 79-85.

Taussky, H. H & E. E. Shor. 1953. A microcolometric method for determination of inorganic phosphorus. J. Biol. Chem. 202(2): 85-675.

Troine, W, S. Kinal, & B. Lubojemska. 2007. Effect of various forms of zinc applied in concentrate mixtures for broiler chickens on its bioavailability as well as meat composition and quality. Poult. J. Food. Nutr. Sci. 57: 577-581.

Vakili, R, A. S. Rashidi, & S. Sobjanirad. 2010. Effects of dietary fat, vitamin E and zinc supplementation on tibia breaking strenght in female broilers under heat stress. Afr. J. Agric. Res. 5(23): 3151-3156.

Vieira, M. M, A. M. L. Riberio, A. M. Kessler, M. A. Kunrath, & V. S. Ledur. 2013. Different sources of dietary zinc for broiler submitted to immunological, nutritional and environmental challange. J. Appl. Poult. Res. 22: 855-861.

Yang, W. L, Y. P. Chen, Y. F. Cheng, X. H. Li, R. Q. Zhang, C. Wen, & Y. M. Zhou. 2016. An evaluation of zinc bearing palygorskite inclusion on the growth performance, mineral content, meat quality and atioxidant status of broilers. Poult. Sci. 95:878-885.

Yu, J, P. Yin, F. H. Liu, G. L. Cheng, K. J. Guo, A. Lu, X. Y. Zhu, W. L. Luan, & J. Q. Xu. 2010. Effect of heat stress on the porcine small intestine: A morphological and gene expression study. Comp. Biochem. Physiol. A Mol. Intergr. Physiol. 156:119-128.

Yu, Y, L. Lu, X. G. Luo, & B. Liu. 2008. Kinetics of zinc absorption by in situ ligated intestinal loops of broilers involved in zinc transportes. Poult. Sci. 87:1146-1155.

Yu, Y, L. Lu, R. L. Wang, L. Xi, X. G. Luo, & B. Liu. 2010. Effect of zinc source and phytate on zinc absoption by in situ ligated intestinal loops of broilers. Poult. Sci. 89:2157-2165.

Zaghari, M, M. Avazkhanllo, & M. Ganjkhanlou. 2015. Reevaluation of male broilerzinc requirement by dose-response trial using practical diet with added exogenus phytase. J. Agr. Sci. Tech. 17: 333-343.

Zhang, Z. Y, G. Q. Jia, J. J. Zuo, Y. Zhang, J. Lei, L. Ren, & D. Y. Feng. 2012. Effect of constant and cyclic heat stess on muscle metabolism and meat quality of broiler breast fillet and thigh meat. Poult. Sci. 91:2931-2937.


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