Effectiveness of the Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as the Colorant, Antioxidant, and Antimicrobial on Beef Sausage

  • Fitri M Manihuruk Study Program of Animal Production and Technology, Faculty of Animal Science, Graduate School, Bogor Agricultural University
  • Tuti Suryati Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
  • Irma Isnafia Arief Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University
Keywords: antimicrobial, antioxidant, beef sausage, red dragon fruit peel extract

Abstract

This study aimed to evaluate the effectiveness of red dragon fruit (Hylocereus polyrhizus) peel extracts addition on beef sausages. Red dragon fruit peel extracts were obtained by maceration using solvent at pH 5. Phytochemical characteristics, total phenols, antioxidant, and antimicrobial activity of the peel extracts were observed. Antioxidant and antimicrobial activities of the extracts were associated with high phytochemical compounds and total phenols contained in the extracts. Red dragon fruit peel extracts with various percentages (0%, 20%, 30%, and 40%) were added on beef sausages, and their physicochemical characteristics, nutrients, antioxidant activity, and microbiological profile were analyzed. The data were analyzed using analysis of variance and Duncan’s multiple range test. Results showed that the addition of red dragon fruit peel extracts significantly reduced texture values, but increased intensity of luminosity, intensity of red color, and intensity of yellow color (P<0.05) beef sausages. It could be concluded that red dragon fruit peel extract containing phytochemical compounds was effective as an antibacterial agent and natural antioxidant. The addition of red dragon fruit peel extracts was effective in increasing the antioxidant activity and decreasing TBARS values. The addition of red dragon fruit peel extract did not affect the reddish colorization of beef sausages, but it was capable of increasing the yellowish colorization on beef sausage.

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References

Adams, M. R. & M. O. Moss. 2008. Food Microbiology. 3rd ed. RSC Publising, Cambridge, UK.

Adnan, L., A. Osman, & A. A. Hamid. 2011. Antioxidant activity of different extracts of red pitaya (Hylocereus polyrhizus) seed. Int. J. Food Prop. 14: 1171-1181. https://doi.org/10.1080/10942911003592787

Amalia, S., S. Wahdaningsih, & E. K. Untari. 2015. Antibacterial activity testing of n-hexane fraction of red dragon (Hylocereus polyrhizus Britton & Rose) fruit peel on Staphylococcus aureus ATCC 25923. Trad. Med. J. 19: 89-94.

Aminlari, M., S. S. Shekarforoush, H. R. Gheisari, & L. Golestan. 2009. Effect of actinidin on the protein solubility, water holding capacity, texture, electrophoretic pattern of beef, and on the quality attributes of a sausage product. J. Food Sci. 74: 221-226. https://doi.org/10.1111/j.1750-3841.2009.01087.x

AOAC. 2005. Official Methods of Analysis of AOAC International. 18th ed. Assoc. Off. Anal. Chem., Arlington.

Arief, I. I., C. Budiman, B. S. L. Jenie, E. Andreas, & A. Yuneni. 2015. Plantaricin IIA-1A5 from Lactobacillus plantarum IIA-1A5 displays bactericidal activity against Staphylococcus aureus. Beneficial Microb. 6: 603-613. https://doi.org/10.3920/BM2014.0064

Arief, I. I., T. Suryati, D. Afiyah, & D. Wardhani. 2014. Physicochemical and organoleptic of beef sausages with teak leaf extract (Tectona grandis) addition as preservative and natural dye. Int. Food Res. J. 21: 2033-2042.

Baxter, H., J. B. Harborne, & G. P. Moss. 1998. Phytochemical Dictionary: A Handbook of Bioactive Compounds from Plants. CRC Press, London, UK.

BSN. 1995. Standar Nasional Indonesia: Sosis Daging (SNI 01-3820:1995). Badan Standardisasi Nasional, Jakarta.

Choi, J. G., O. H. Kang, Y. S. Lee, H. S. Chae, Y. C. Oh, O. O. Brice, M. S. Kim, D. H. Sohn, H. S. Kim, H. Park, D. W. Shin, J. R. Rho, & D. Y. Kwon. 2011. In vitro and in vivo antibacterial activity of punica granatum peel ethanol extract against Salmonella. Evid. Based Comp. Alternat. Med. 2011: 1-8. https://doi.org/10.1093/ecam/nen085

Faridah, A., R. Holinesti, & D. Syukri. 2015. Betalains from red pitaya peel (Hylocereus polyrhizus): extraction, spectrophotometric and HPLC-DAD identification, bioactivity and toxicity screening. Pakistan J. Nutr. 14: 976-982. https://doi.org/10.3923/pjn.2015.976.982

FDA. 1998. Bacteriological Analytical Manual. 8th ed. Assoc. Off. Anal. Chem., Arlington.

Fidrianny, I., M. Harnovi, & M. Insanu. 2014. Evaluation of antioxidant activities from various extracts of sweet orange peels using DPPH, FRAP assays and correlation with phenolic, flavonoid, carotenoid content. Asian J. Pharmaceutical & Clinical Res. 7: 186-190.

Harivaindaran, K., O. Rebecca, & S. Chandran. 2008. Study of optimal temperature, pH and stability of dragon fruit (Hylocereus polyrhizus). Pakistan J. Biol. Sci. 11: 2259-2263. https://doi.org/10.3923/pjbs.2008.2259.2263

Herbach, K., F. Stintzing, & R. Carle. 2004. Impact of thermal treatment on color and pigment pattern of red beet (Beta vulgaris L.) preparations. J. Food Sci. 69: 491-498. https://doi.org/10.1111/j.1365-2621.2004.tb10994.x

Honikel, K. O. 2008. The use and control of nitrate and nitrite for the processing of meat products. Meat Sci. 78: 68-76. https://doi.org/10.1016/j.meatsci.2007.05.030

Jamilah, B., C. E. Shu, M. A. Kharidah, & A. Noranizan. 2011. Physico-chemical characteristics of red pitaya (Hylocereus polyrhizus) peel. Int. Food Res. J. 18: 279-286.

Jay, J. M. 2012. Modern Food Microbiology. 5th ed. Aspen Publisher Inc. Gaithersburg, Maryland.

Jongberg, S., M. A. Torngren, A. Gunvig, L. H. Skibsted, & M. N. Lund. 2013. Effect of green tea or rosemary extract on protein oxidation in Bologna type sausages prepared from oxidatively stressed pork. Meat Sci. 93: 538-546. https://doi.org/10.1016/j.meatsci.2012.11.005

Kumar, Y., D. N. Yadav, T. Ahmad, & K. Narsaiah. 2015. Recent trends in the use of natural antioxidants for meat and meat products. Compr. Rev. Food Sci. & Food Safety 14: 796-812. https://doi.org/10.1111/1541-4337.12156

Lourith, N. & M. Kanlayavattanakul. 2013. Antioxidant and stability of dragon fruit peel colour. Agro. Food Ind. Hi-Tech 24: 56-58.

Luo, H., Y. Cai, Z. Peng, T. Liu, & S. Yang. 2014. Chemical composition and in vitro evaluation of the cytotoxic and antioxidant activities of supercritical carbon dioxide extracts of pitaya (dragon fruit) peel. Chem. Central J. 8: 1-7. https://doi.org/10.1186/1752-153X-8-1

Nurliyana, R., I. Syed Osman, I. Syed Zahir, K. Mustapha Suleiman, M. R. Aisyah, & K. Kamarul Rahim. 2010. Antioxidant study of pulps and peels of dragon fruits: a comparative study. Int. Food Res. J. 17: 367-375.

Nurmahani, M. M., A. Osman, A. A. Hamid, F. M. Ghazali, & M. P. Dek. 2012. Antibacterial property of Hylocereus polyrhizus and Hylocereus undatus peel extracts. Int. Food Res. J. 19: 77-84.

Nurul, H., T. Alistair, H. Lim, & I. Noryati. 2010. Quality characteristics of Malaysian commercial beef frankfurters. Int. Food Res. J 17: 469-476.

Rohin, M. A. K., A. Bakar, C. Abdullah, & A. M. Ali. 2012. Antibacterial activity of flesh and peel methanol fractions of red pitaya, white pitaya and papaya on selected food microorganisms. Int. J. Pharmacy & Pharmaceutical Sci. 4: 185-190.

Sorensen, G. & S. S. Jorgensen. 1996. A critical examination of some experimental variables in the 2-thiobarbituric acid (TBA) test for lipid oxidation in meat products. Zeitschrift fur Lebensmittel-Untersuchung und Forschung. 202:205-210.

Suryati, T., M. Astawan, H. N. Lioe, T. Wresdiyati, & S. Usmiati. 2014. Nitrite residue and malonaldehyde reduction in dendeng-Indonesian dried meat-influenced by spices, curing methods and precooking preparation. Meat Sci. 96: 1403-1408. https://doi.org/10.1016/j.meatsci.2013.11.023

Tahera, J., F. Feroz, J. D. Senjuti, K. K. Das, & R. Noor. 2014. Demonstration of anti-bacterial activity of commonly available fruit extracts in Dhaka, Bangladesh. American J. Microb. Res. 2: 68-73. https://doi.org/10.12691/ajmr-2-2-5

Tangkanakul, P., P. Auttaviboonkul, B. Niyomwit, N. Lowvitoon, P. Charoenthamawat, & G. Trakoontivakorn. 2009. Antioxidant capacity, total phenolic content and nutritional composition of Asian foods after thermal processing. Int. Food Res. J. 16: 571-580.

Totosaus, A. 2009. Handbook of Processed Meats and Poultry Analysis: Colorants. L. M. L. Nollet, & F. Toldra (Eds). CRC Press, London, UK.

Woo, K. K., F. H. Ngou, L. S. Ngo, W. K. Soong, & P. Y. Tang. 2011. Stability of betalain pigment from red dragon fruit (Hylocereus polyrhyzus). Am. J. Food Technol. 6:140-148.

Wu, L. C., H. W. Hsu, Y. C. Chen, C. C. Chiu, Y. I. Lin, & J. A. A. Ho. 2006. Antioxidant and antiproliferative activities of red pitaya. Food Chem. 95: 319-327. https://doi.org/10.1016/j.foodchem.2005.01.002

Youssef, M. K. & S. Barbut. 2010. Physicochemical effects of the lipid phase and protein level on meat emulsion stability, texture, and microstructure. J. Food Sci. 75: 108-114. https://doi.org/10.1111/j.1750-3841.2009.01475.x

Zobra, O., H. Y. Gokalp, H. Yetim, & H. W. Ockerman. 1993. Model system evaluations of the effects of different levels of K2HPO4, NaCl and oil temperature on emulsion stability and viscosity of fresh and frozen Turkish style meat emulsion. Meat Sci. 34: 145-161. https://doi.org/10.1016/0309-1740(93)90024-C

Published
2017-04-25