Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens

H. Julendra, Ade Erma Suryani, Lusty Istiqomah, Ema Damayanti, Muslih Anwar, Naditya Fitriani

Abstract


The aim of the study was to identify the cholesterol-lowering activity of indigenous lactic acid bacteria isolated from the small intestine, cecum, and colon of Indonesian native chickens and evaluated for bile salt hydrolase (BSH) activity in vitro by using MRS media added taurodeoxycholic acid (TDCA) and CaCl2. The quantitative measurement of cholesterol-lowering activity of LAB was investigated by using soluble cholesterol containing MRS broth (100 µg/mL of cholesterin) and incubated at 37 °C for 48 h. Cholesterol content in supernatant was analyzed using microplate reader. The highest percentage of cholesterol reduction found in isolates from colon of native chicken with the value of 17.43% and identified as Lactobacillus plantarum. Based on phylogenetic tree analysis, this isolate was closely related to L. plantarum strain LGFCP4 (accession number KM199683.1) isolated from GIT of Guinea fowl from India. It could be concluded that L. plantarum AKK-30 had cholesterol-lowering activity.

Keywords


lactic acid bacteria (LAB); cholesterol; bile salt hydrolase (BSH); native chicken

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References


Adams, M. R. & R. Nout. 2001. Fermentation and food safety. Aspen Publishers, Washington DC.

Agaliya, P. J. & K. Jeevaratnam. 2012. Screening of Lactobacillus plantarum isolated from fermented idli for probiotics properties. Afr. J. Biotechnol. 11: 12856-12864.

Becher, P., M. Orlich, A. D. Shannon, G. Horner, M. KÖnig, & H. J. Thiel. 1997. Phylogenetic analysis of pestiviruses from domestic and wild ruminants. J. General Virol. 78: 1357-1366. https://doi.org/10.1099/0022-1317-78-6-1357

Cenesiz, S., H. Yaman, A. Ozcan, & G. Karademr. 2008. Effect of kefir as probiotic on serum cholesterol, total lipid, aspartate amino transferase and alanina amino transferase activities in broiler chicks. Med. Weter. 64:168-170.

Cobos, M. A., A. L. de Coss, N. D. Ramirez, S. S. Gonzales, & R. F. Cerrato. 2011. Pediococcus acidilactici isolated from the rumen of lambs with rumen acidosis, 16S rRNA identification and sensibility to monensian and lasalocid. Res. Vet. Si. 90: 26-30. https://doi.org/10.1016/j.rvsc.2010.05.006

Coenye, T. & P. Vandamme. 2003. Extracting phylogenetic information from whole-genome sequencing projects: the lactic acid bacteria as a test case. Microbiology 149: 3507-3517. https://doi.org/10.1099/mic.0.26515-0

Damayanti, E., H. Herdian, M. Angwar, A. Febrisiantosa & L. Istiqomah. 2012. Lactic acid bacterial screening from gastrointestinal digestive tract of native broiler chicken for probiotic candidate purposes. J. Indonesian Trop. Anim. Agric. 37: 168-175. https://doi.org/10.14710/jitaa.37.3.168-175

de Ross, N. M. & M. B. Katan. 2000. Effect of probiotic bacteria on diarhea, lipid metabolism, and carcinogenesis: A review of paper published between 1988 and 1998. American Journal of Clinical Nutrition. 71: 405-411.

Guo, L-D., L-.J Yang, & G-C. Huo. 2011. Cholesterol removal by Lactobacillus plantarum isolated from homemade fermented cream in inner Mongolia of China. Czech J. Food Sci. 29: 219-225.

Gong, J., W. Si, R. J. Foster, R. Huang, H. Yu, Y. Yin, C. Yang, & Y. Han. 2007. 16S rRNA Gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiol. Ecol. 59: 147-157. https://doi.org/10.1111/j.1574-6941.2006.00193.x

Harimurti, S., E. S. Rahayu, Nasroedin, & Kurniasih. 2005. Bakteri asam laktat dari intestin ayam sebagai agensia probiotik. Anim. Prod. 9: 82-91.

Heravi, R. M., H. Kermanshahi, M. Sankian, M. R. Nassiri, A. H. Moussavi, L. R. Nasiraii, & A. R. Varasteh. 2011. Screening of lactobacilli bacteria isolated from gastrointestinal tract of broiler chickens for their use as probiotic. African J. Microbiol. Res. 5: 1858-1868.

Huang, Y., X. Wang, J. Wang, F. Wu, Y. Sui, L. Yang, & Z. Wang. 2013. Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity. J. Dairy Sci. 96: 2746-2753. https://doi.org/10.3168/jds.2012-6123

Ismoyowati, I. & T. Widiyastuti. 2003. Fat and cholesterol content of breast and thigh meat of local fowls. J. Animal Production 5: 79-82.

Jannah, S. N., A. Dinoto, K. G. Wiryawan, & I. Rusmana. 2014. Characteristics of lactic acid bacteria isolated from gastrointestinal tract of cemani chicken and their potential use as probiotics. Med. Pet. 37:182-189. https://doi.org/10.5398/medpet.2014.37.3.182

Kleerebezem, M. & J. Hugenholtz. 2003. Metabolic pathway engineering in lactic acid bacteria. Curr. Opin. Biotechnol. 14:232–237. https://doi.org/10.1016/S0958-1669(03)00033-8

Krieg, N. R., J. T. Staley, D. R. Brown, B. P. Hedlund, B. J. Paster, N. L. Ward, W. Ludwig, & W. B. Whitman. 2010. Bergey’s Manual of Systematic Bacteriology. 2nd Ed. Springer, USA.

Kumar, M., R. Nagpal, R. Kumar, R. Hemalatha, V. Verma, A. Kumar, C. Chakraborty, B. Singh, F. Marotta, S. Jain, & H. Yadav. 2012. Cholesterol-lowering probiotics as potential biotherapeutics for metabolic diseases. Experimental Diabetes Research. Vol. 2012: 1-14. https://doi.org/10.1155/2012/902917

Lim, H. J., S. Y. Kim, & W. K. Lee. 2004. Isolation of lactic acid bacteria from human intestine for probiotic use. J. Vet. Sci. 5: 391-395.

Ooi, L. G. & M. T. Liong. 2010. Cholesterol-lowering effects of probiotics and prebiotics: a review of in vivo and in vitro findings. Int. J. Mol. Sci. 11: 2499-2522. https://doi.org/10.3390/ijms11062499

Seeley, R. R., D. S. Trent, & T. Philip. 2000. Anatomy and Physiology. 7thed. McGraw-Hill Co, New York.

Shazali N., H. L. Foo, T. C. Loh, D. W. Choe, & R. A. Rahim. 2014. Prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in Malaysia. Gut Pathogens 6: 1-7. https://doi.org/10.1186/1757-4749-6-1

Sirilun, S., C. Chaiyasut, D. Kantachote, & P. Luxananil. 2010. Characterisation of non human origin probiotic Lactobacillus plantarum with cholesterol-lowering property. African J. Microbiol. Res. 14: 994-1000.

Stackebrandt, E. & Ebers. 2006. Taxonomic parameters revisited: “Tarnished Gold Standars”. Microbiol. Today 33: 152-155.

Tomaro-Duchesneau C., M. L. Jones, D. Shah, P. Jain, A. Saha, & S. Prakash. 2014. Cholesterol assimilation by Lactobacillus probiotic bacteria: an in vitro investigation. BioMed Res. Int. Article ID 380316, 9 pages.

Torshizi M.K., S. Rahimi, N. Mojgani, S. Esmaeilkhanian, & J. L. Grimes. 2008. Screening of indigenous strains of lactic acid bacteria for development of a probiotic for poultry. Asian-Aust. J. Anim. Sci. 21: 1495-500.




DOI: http://dx.doi.org/10.5398/medpet.2017.40.1.35

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