Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment

Synbiotic Microcapsule Dietary Supplementation for the Health Status Improvement of the White Shrimp

Abstract

This study aimed to evaluate the effectivity of microencapsulated synbiotic (MS), Bacillus sp. NP5 and mannan oligosaccharide (MOS) dietary in different feeding frequencies in Pacific white shrimp culture field experiment. The MS was administered as a feed supplementation to enhance the immunity for prevention against co-infection with WSSV (White Spot Syndrome Virus) and Vibrio harveyi. The synbiotic was microencapsulated by the spray dryer method. Shrimps were reared in the floating net cages in the pond. Treatments included the administration of MS at different frequencies i.e, daily (A), twice a week (B), once a week (C), and without MS supplementation (consisted of negative and positive controls) with a feeding rate of 6% of shrimp biomass (5 times a day). During the challenge trial, shrimps were removed and further reared in plastic tanks, for 7 days. The shrimps (except negative control treatment) were intramuscularly injected by WSSV filtrate at the infective dosage of 10-4 copies.ml-1. Twenty four hours after WSSV injection the shrimps were immersed in the water-containing cells suspension of V. harveyi at the cell’s population dosage of 106 CFU.ml-1. Immune responses were observed for 7 days after experimental infection. The shrimps that have been treated with daily MS supplementation (A) showed better immune responses i.e., total haemocyte counts, phenoloxidase, respiratory burst, and the lower pathogenic cells abundance in the intestine compared to other treatments groups.

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Author Biographies

Andreas Tambun, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680
  • Aquaculture Science, Master’s degree, Post Graduate Study, IPB University
  • Ranking A  , National Accreditation
Widanarni Widanarni, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Dramaga, West Java, Indonesia 16680
  • Department of Aquaculture, Faculty of Fishery and Marine Science, IPB University
  • A Ranking, National Accrediation
Usamah Afiff, Department of Animal Disease and Veterinary Public Health, IPB University
  • Department of Animal Disease and Veterinary Public Health, IPB University
  • A Ranking, National Accreditation
Published
2022-08-30
How to Cite
Munti Yuhana, TambunA., WidanarniW., & AfiffU. (2022). Synbiotic microcapsule dietary supplementation for prevention against co-infection diseases in Pacific white shrimp: a limited field experiment: Synbiotic Microcapsule Dietary Supplementation for the Health Status Improvement of the White Shrimp. Jurnal Akuakultur Indonesia, 21(2), 125-132. https://doi.org/10.19027/jai.21.2.125-132