Spesies Meloidogyne penyebab umbi berbintil kentang pada tiga sentra produksi di Sumatra

  • Ilmi Hamidi IPB University
  • Supramana Department of Plant Protection, IPB University
  • Kikin Hamzah Mutaqin Department of Plant Protection, IPB University
  • Fitrianingrum Kurniawati Department of Plant Protection, IPB University
Keywords: CO1, morphology, morphometry, perineal pattern, phylogenetic analysis

Abstract

Meloidogyne species, the pimple-like knot pathogen of potato tuber in three production centers in Sumatra

The root-knot nematode (Meloidogyne spp.) is the cause of pimple-like swelling symptoms, which results in a decrease in the quality and quantity of potato production in the Sumatra region. Identification of the Meloidogyne species is necessary for designing its effective control strategies. This study aimed to detect and identify Meloidogyne species in potatoes morphologically, morphometry, and molecularly. Potato tubers with pimple-like swelling symptoms, tuber shape malformations, uneven tuber surface, and corrugated tuber surface were collected from 3 potato production centers in Sumatra, i.e., Karo (North Sumatra), Solok (West Sumatra), and Kerinci (Jambi). Nematode extraction was conducted by a surgical technique of spotted tuber tissue. Morphological identification was conducted based on the perineal patterns of females. Morphometric measurements were carried out on juvenile 2 based on the de Man formula. Molecular identification was conducted by PCR technique, followed by nucleotide sequencing and phylogenetic analysis. Three species of Meloidogyne identified were Meloidogyne arenariaM. incognita, and M. javanica. DNA amplification using a CO1-specific primer successfully amplified DNA bands of ±360 bp for M. arenaria, ±326 bp for M. incognita, and ±170 bp for M. javanica. The sequencing results showed that M. incognita isolates from Karo-Indonesia were very closely related to M. incognita isolates from China, the United States, Vietnam, the United Kingdom, Brazil, and South Africa. M. javanica isolates from Solok-Indonesia were very closely related to M. javanica from the United States, Africa, China, Germany, and the United Kingdom.

Downloads

Download data is not yet available.

References

Adam MAM, Phillips MS, Blok VC. 2007. Molecular diagnostic key for identification of single juveniles of seven common and economically import species of root-knot nematode (Meloidogyne spp.). Plant Pathology. 56:190–197. DOI: https://doi.org/10.1111/j.1365-3059.2006.01455.x.

Aprilyani, Supramana, Suastika G. 2015. Meloidogyne incognita penyebab umbi berbintil pada kentang di beberapa sentra produksi kentang di Jawa. Jurnal Fitopatologi Indonesia. 11(5):143–149. DOI: https://doi.org/10.14692/jfi.11.5.143.

Eisenback JD, Hirschman H, Sasser JN, Triantaphyllou AC. 1981. A Guide to The Four Most Common Spesies of Root-Knot Nematodes (Meloidogyne spp.), With a Pictural Key. Washington DC (US): Cooperative Publication Department of Plant Pathology an US Agency International Development.

Ghaderi R, Karrsen G. 2020. An updated checklist of Meloidogyne Göldi, 1887 species, with a diagnostic compendium for second-stage juveniles and males. Journal of Crop Protection. 9(2):183–193.

Holterman M, van der Wurff A, van den Elsen S, van Megen H, Bongers T, Holovachov O, Bakker J, Helder J. 2006. Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution. 23(9):1792–800. DOI: https://doi.org/10.1093/molbev/msl044.

Hunt DJ, Handoo ZA. 2009. Taxonomy, identification and principal species. Di dalam: Perry RN, Moens M, Starr JL, editor. Root-knot Nematodes. Maryland (US): CAB International. Hlm 55–88. DOI: https://doi.org/10.1079/9781845934927.0055.

Janssen T, Karssen G, Topalovic O, Coyne D, Bert W. 2017. Integrative taxonomy of root-knot nematodes reveals multiple independent origins of mitotic parthenogenesis. Plos One. 12(3): e0172190. DOI: https://doi.org/10.1371/journal.pone.0172190.

Khan MR, Handoo ZA, Rao U, Rao SB, Prasad JS. 2012. Observations on the foliar nematode Aphelenchoides besseyi, infecting tuberose and rice in India. Journal of Nematology. 44(4):391–398.

Kurniawati F, Supramana, Munif A. Spesies Meloidogyne penyebab puru akar pada seledri di Pacet, Cianjur, Jawa Barat. Jurnal Fitopatologi Indonesia. 13(1):26–30. DOI: https://doi.org/10.14692/jfi.13.1.26.

Lisnawita, Nainggolan HEA, Wardhani AK. 2014. Identifikasi spesies nematoda puru akar (Meloidogyne spp.) pada tanaman kentang di Kabupaten Karo, Sumatra Utara. Di dalam: Prosiding Seminar Nasional dan Rapat Tahunan Dekan Bidang Ilmu Pertanian BKS-PTN Wilayah Barat; 19–21 Agustus 2014; Bandar Lampung (ID): Fakultas Pertanian Universitas Lampung. Hlm 828–831.

Mirsam H, Supramana, Suastika G. 2015. Deteksi dan identifikasi spesies Meloidogyne pada tanaman wortel dari Dataran Tinggi Malino, Gowa, Sulawesi Selatan. Jurnal Fitopatologi Indonesia. 11(1):1–8. DOI: https://doi.org/10.14692/jfi.11.1.1.

Moens M, Perry RN, Starr JL. 2009. Meloidogyne species, a Diverse Group of Novel and Important Plant Parasites. London (UK): British Library.

Pratiwi NWK, Auly FE, Amrulloh R, Kurniawati F. 2020. Deteksi dan Identifikasi Nematoda Puru Akar (Meloidogyne spp.) pada Tanaman Bit Menggunakan Metode DNA Barcoding. Jurnal Fitopatologi Indonesia. 16(1):1–8. DOI: https://doi.org/10.14692/jfi.16.1.1-8.

Rusinque L, Inácio ML, Mota M dan Nóbrega F. Morphological, biochemical and molecular characterisation of Meloidogyne javanica, from North Portugal, in tomato. Revista de Ciências Agrárias. 41(1): 193–198. DOI: https://doi.org/10.19084/RCA17078.

Ryss AY. 2017. A simple express technique to process nematodes for collection slide mounts. Journal of Nematology. 49(1):27–32. DOI: https://doi.org/10.21307/jofnem-2017-043.

Utami BS, Supramana, Giyanto. 2017. Deteksi dan identifikasi spesies Meloidogyne penyebab umbi berbintil pada kentang asal Sulawesi Utara. Jurnal Fitopatologi Indonesia. 13(3):98–104. DOI: https://doi.org/10.14692/jfi.13.3.98.

Zuckerman BM, Mai WF, Harrison MB. 1985. Plant Nematology, Laboratory Manual. Massachusetts (US): The University of Massachussett.

Published
2022-08-05
How to Cite
HamidiI., Supramana, MutaqinK. H., & KurniawatiF. (2022). Spesies Meloidogyne penyebab umbi berbintil kentang pada tiga sentra produksi di Sumatra. Jurnal Fitopatologi Indonesia, 18(2), 66-74. https://doi.org/10.14692/jfi.18.2.66-74
Section
Articles